This is just a guideline on the perfect bitch cycle. Every female can be different. It might take her longer or shorter to ovulate. They might be slow at the beginning and then speed up getting out of the breeding range very fast. Ovulation will occur anytime when progesterone is between 4ng/ml and 10ng/ml. The perfect bitch will ovulate when her progesterone levels reach 5ng/ml. The initial vaginal artificial insemination is recommended to be conducted 48 hours post-ovulation when the progesterone level attains 5 ng/ml. Transcervical insemination (TCI) is advised to be carried out 72 hours following ovulation, regardless of the progesterone level. If two TCIs are planned, they should be scheduled for 48 hours and 72 hours after ovulation. A surgical implant should be performed 72 hours after ovulation no matter what the progesterone level is. If you get the first reading over 10ng/ml and you don't know when the bitch ovulated, you need to confirm fertility with cytology and perform either a TCI or a surgical implant. While the eggs might still be fertile, the cervix might have already closed, and vaginal insemination will be unsuccessful. If you get readings that do not make sense, then re-test. Sperm survival times in the bitch vary depending on how the semen was preserved. Fresh semen lives 4-6 days in the bitch, chilled semen lives 3 days in the bitch, and frozen semen lives 12 hours in the bitch.
INTERPRETATION OF PROGESTERONE LEVELS: This is just a guideline on the perfect bitch cycle. Every female can be different. It might take her longer or shorter to ovulate. They might be slow at the beginning and then speed up getting out of the breeding range very fast. Ovulation will occur anytime when progesterone is between 4ng/ml and 10ng/ml. The perfect bitch will ovulate when her progesterone levels reach 5ng/ml. The first vaginal artificial insemination should be performed 48 hours after ovulation when the progesterone reaches 5 ng/ml. Transcervical insemination (TCI) should be performed 72 hours after ovulation no matter what the progesterone level is. If you are doing two TCIs they should be performed 48 hours and 72 hours after ovulation. A surgical implant should be performed 72 hours after ovulation no matter what the progesterone level is. If you get the first reading over 10ng/ml and you don't know when the bitch ovulated, you need to confirm fertility with cytology and perform either a TCI or a surgical implant. While the eggs might still be fertile, the cervix might have already closed, and vaginal insemination will be unsuccessful. If you get readings that do not make sense, then re-test. Sperm survival times in the bitch vary depending on how the semen was preserved. Fresh semen lives 4-6 days in the bitch, chilled semen lives 3 days in the bitch, and frozen semen lives 12 hours in the bitch. LH surge occurs 24 - 48 hours before ovulation. There is a test available for this. It must be done daily. INTERPRETATION OF PROGESTERONE LEVELS: 0.1-0.9ng/ml ~ 0-2 nmol/LEARLY READINGS 1.0-1.9ng/ml ~ 3-6 nmol/LRE-TEST IN 3 DAYS 2.0-3.9ng/ml ~ 7-12 nmol/LRE-TEST IN 2 DAYS 3.0-4.9ng/ml ~ 7-12 nmol/L BREED IN 2 DAYS RE-TEST IN 1-2 DAYS SHIP TOMORROW 5.0 ng/ml ~ 13-31 nmol/LOVULATION HAS OR WILL OCCUR SHORTLY SHIP TOMORROW BREED IN 1-2 DAYS 5.0-7.9ng/ml ~ 32-64 nmol/L START BREEDING 8.0-10.0 ng/ml ~ 32-64 nmol/L BEST TIME TO BREED 10.0-15.0 ng/ml ~ 65-90 nmol/LBEST TIME TO BREED SURGICAL >15.0 ng/ml ~ >90 nmol/LIT MIGHT BE LATE. DO A CYTOLOGY Progesterone in ng/ml x 3.18 = Progesterone in nmol/L Progesterone in nmol/L ÷ 3.18 = Progesterone in ng/ml Disclaimer The information on this page is believed to be accurate, but in no way, it constitutes professional advice. Consult your theriontologist for the best plan for your bitch.